The Different Effect of Decellularized Myocardial Matrix Hydrogel and Decellularized Small Intestinal Submucosa Matrix Hydrogel on Cardiomyocytes and Ischemic Heart

Injectable decellularized matrix hydrogels derived from either myocardium or small intestinal submucosa (pDMYO-gel, pDSIS-gel) have been successfully used for myocardial injury repair. However, the relationship between tissue-specific biological functions and protein composition in these two materials is not clear yet. In this study, composition, mechanical properties, morphology of their effects on behavior neonatal rat cardiomyocytes (NRCMs) human umbilical vein endothelial cells (HUVECs), are investigated. The results show that pDMYO-gel more conducive to growth, adhesion, spreading, maintenance normal NRCM beating, due its higher proportion extracellular (ECM) glycoproteins (49.55%) some unique functional proteins such as annexin-6 (ANXA6), agrin (AGRN), cathepsin D (CTSD) galectin-1 (LGALS1), whereas pDSIS-gel proliferation HUVECs. Animal study shows has a better effect improving cardiac function, inhibiting fibrosis maintaining ventricular wall thickness acute infarction models vivo. Therefore, it proposed injectable hydrogel may be suitable recovery injuries.